Header Banner
wonderhowto.mark.png
Gadget Hacks Next Reality Food Hacks Null Byte The Secret Yumiverse Invisiverse Macgyverisms Mind Hacks Mad Science Lock Picking Driverless

How to Apply C. elegans dopamine neuron degeneration assay

"How to Apply C. elegans dopamine neuron degeneration assay" cover image

Check out this science video tutorial on how to apply a C. elegans dopamine neuron degeneration assay for the validation of potential Parkinson's Disease genes.





Improvements to the diagnosis and treatment of Parkinson's disease (PD) are dependent upon knowledge about susceptibility factors that render populations at risk. In the process of attempting to identify novel genetic factors associated with PD, scientists have generated many lists of candidate genes, polymorphisms, and proteins that represent important advances, but these leads remain mechanistically undefined. Our work is aimed toward significantly narrowing such lists by exploiting the advantages of a simple animal model system. While humans have billions of neurons, the microscopic roundworm Caenorhabditis elegans has precisely 302, of which only eight produce dopamine (DA) in hemaphrodites. Expression of a human gene encoding the PD-associated protein, alpha-synuclein, in C. elegans DA neurons results in dosage and age-dependent neurodegeneration. Worms expressing human alpha-synuclein in DA neurons are isogenic and express both GFP and human alpha-synuclein under the DA transporter promoter (Pdat-1). The presence of GFP serves as a readily visualized marker for following DA neurodegeneration in these animals. We initially demonstrated that alpha-synuclein-induced DA neurodegeneration could be rescued in these animals by torsinA, a protein with molecular chaperone activity. Further, candidate PD-related genes identified in our lab via large-scale RNAi screening efforts using an alpha-synuclein misfolding assay were then over-expressed in C. elegans DA neurons. We determined that five of seven genes tested represented significant candidate modulators of PD as they rescued alpha-synuclein-induced DA neurodegeneration.

URL Preview
Apply C. elegans dopamine neuron degeneration assay

Click through to watch this video on jove.com

External Content
www.jove.com

Apple's iOS 26 and iPadOS 26 updates are packed with new features, and you can try them before almost everyone else. First, check Gadget Hacks' list of supported iPhone and iPad models, then follow the step-by-step guide to install the iOS/iPadOS 26 beta — no paid developer account required.

Related Articles

Comments

No Comments Exist

Be the first, drop a comment!