The Department of Biological Sciences at the University of Alberta brings you this video tutorial specifically relevant to your student laboratory courses, specifically microbiology. If you're a student at any school of biology, this information will be helpful for learning how to perform agarose gel electrophoresis in the lab.
Part A: Set up the gel apparatus. Notice that we test the depth of the wells with the spacer - the small side will fit under the teeth of the comb easily while the larger side does not.
Part B: Obtain some molten agarose, swirl gently (avoid bubbles), making sure the aragose is warm to the touch but not too hot. Pour the molten agarose in apparatus, let cool.
Part C: Remove comb and put gel into the running box. Pour buffer on top of the gel.
Part D: Load samples carefully and start electrophoresis.
Part E: Notice how the dye has been separated into three bands. In this experiment, the direction of travel was from right (the cathode) to left (the anode).
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